New Products – Indicating FTA DMPK cards for use with colorless samples such as plasma, urine and CSF.(See below.)



New Products – Indicating FTA DMPK cards for use with colorless samples such as plasma, urine and CSF. (See below.)

Dried blood spot microvolume sampling for DMPK


Drug metabolism (DM), pharmacokinetic (PK) and toxicokinetic (TK) studies provide crucial insight into how drug candidates behave in the body and are therefore critical steps in drug development.

These types of analysis traditionally require large volumes of blood (generally 100 - 500 μl per subject per time point) to provide sufficient plasma volume for quantitative bioanalysis.  Because only a limited blood volume can be drawn from each animal the number of serial samples is restricted and composite sampling must be used.  This use results in lower quality PK data and an increase in the number of animals required.  The large volumes also make it difficult to conduct studies in juvenile subjects.

Additionally, plasma needs to be isolated from whole blood and prepared for bioanalysis using solid phase extraction, liquid-liquid extraction or protein precipitation.  This time-consuming process limits throughput and consequently how many samples can be tested.

Finally, there are practical challenges with shipping and storing blood samples that require controlled handling and frozen transportation and storage.

The dried blood spot (DBS) method is an alternative technique that overcomes these drawbacks.

DBS microvolume sampling using specialized Whatman media from GE Healthcare has been shown to be both precise and accurate for a variety of compounds from different structural classes with acceptable inter- and intra-assay variability.  It is now being routinely employed in PK/TK studies. (See Supporting Documentation below for a list of some users.)  The FTA® DMPK-A and FTA DMPK-B cards lyse cells and denature proteins on contact. Samples can be shipped and stored at ambient temperature and long-term stability has been demonstrated for analytes and metabolites sensitive to plasma enzymes.

Spot.  Extract.  Analyze.

    Apply blood to card and let dry.   Ship and store as needed at  ambient temperature.

    Punch out sample disc and        elute with solvent.

    Analyze solution by HPLC-MS/MS.

Features and Benefits

Lower blood volumes

    DBS microvolume sampling requires only 10 to 20 μl per sample.

    Fewer experimental animals are required because Toxicokinetic data are derived from Toxicology animals, thereby eliminating satellite colonies.

    More consistent data are obtained through more serial sampling from individual animals and less reliance on composite data.

    Juvenile animals and humans can be studied.

 Simpler and safer processing

    The 3-step DBS procedure is much more straightforward than the cumbersome centrifugation, isolation and cleanup of plasma.

    On-substrate cleanup has the potential to convey greater analyte stability, especially for enzyme-sensitive compounds.

 Easier storage and transportation logistics

    Room temperature stability saves on cost of dry ice shipments.

    Shipping without dry ice allows more remote sampling, particularly important for human clinical trail samples.

 Cost savings

    Several of the benefits listed above, particularly reduced animal use and reduced shipping of samples on dry ice, can reduce drug development costs.

Indicating FTA-DMPK Cards

These indicating cards offer the same benefits as our standard dried blood spot technology, but now for clear biosamples such as plasma, urine and cerebrospinal fluid.

When a sample is applied the blue dye is displaced, leaving a lighter-colored area for easy identification.

Reference:  M Barfield and R Wheller (2011) Use of Dried Plasma Spots in the Determination of Pharmacokinetics in Clinical Studies: Validation of a Quantitative Bioanalytical Method. Analytical Chemistry 83 (1), 118-124. 

Spot.  Extract.  Analyze.

  • Apply blood to card and let dry.   Ship and store as needed at  ambient temperature.
  • Punch out sample disc and        elute with solvent.
  • Analyze solution by HPLC-MS/MS.



Indicating FTA-DMPK Cards

Catalog Number     Description

FTA DMPK Cards, white, for blood or other samples

WB129241     FTA DMPK-A Cards, 100/pk
WB129242     FTA DMPK-B Cards, 100/pk
WB129243     FTA DMPK-C Cards, 100/pk
WB129248     FTA DMPK Starter Pack, 45/pk (15 x A, B and C), 3mm Uni-Core Punch and small cutting mat

Punching and Drying Accessories

WB100039     Harris Uni-Core Punch, 3mm, 4/pk with two 2.5" x 3" cutting mats
WB100040     Harris Uni-Core Punch, 6mm, 4/pk with two 2.5" x 3"cutting mats
WB100038     Harris Micro Punch, 3mm, 1/pk with one 5.9" x 7.9" cutting mat
WB100042     Replacement Tip for Micro Punch, 3mm, 1/pk
WB100020     Cutting Mat, large, 5.9" x 7.9"
10537173     Dry Rak (without Velcro), holds 11 cards, 10/pk
10539521     Dry Rak (with Velcro), holds 11 cards, 10/pk

Storage and Shipping Accessories

10548232     Plastic Ziploc Storage Bags, 4"x6", 100/pk
10534321     Foil Barrier Ziploc Bags, for cards 95 x 130 mm max, 100/pk
WB100003     Desiccant Packets, Indicating, 1g, 1000/pk
10548236     Glassine Envelopes, 3.25" x 4.88", 100/pk

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