FTA Elute

FTA Elute Technology 
Collect, Transport, Archive and Isolate Nucleic Acids – All at Room Temperature.

FTA Elute Cards utilize patented Whatman FTA technology that simplifies the handling and processing of nucleic acids. DNA is eluted in an easy step, providing you with DNA in solution for your amplification needs.

The FTA Elute matrix is chemically treated with proprietary reagents that lyse cells upon contact causing the release of nucleic acids. DNA is recovered from the FTA Elute matrix through a simplified elution process using water and heat. Inhibitory components, such as hemoglobin, are retained on the FTA Elute matrix.

The Indicating FTA Elute matrix is the same chemistry as FTA Elute with an indicating dye that changes from purple to white upon application of a colorless sample such as saliva, urine, buccal cell swabs and cultured cells.

Advantages and benefits

  • Samples can be collected, shipped, and preserved at room temperature: Eliminates high costs associated with shipping samples on ice and with laboratory freezer storage requirements.
  • Sample processing time to isolate DNA is 15 to 30 minutes: Eliminates lengthy and multiple step isolation procedure (4 to 16 hours).
  • Sample processing requires a simple hot water elution procedure to isolate DNA: Eliminates cost of using a purification kit.
  • Sample volume requirements are minimal: 12 to 40 µl per collection area: Eliminates venous blood samples and large blood volume handling/processing. Eliminates the need for venipuncture equipment and a medical attendant at the site of collection.
  • Hemoglobin, a known PCR inhibitor, is bound to the FTA Elute matrix: DNA is recovered in solution free of PCR inhibitors.

Collect and isolate samples quickly and easily

Procedure outline
  1. Apply sample to FTA Elute matrix; dry thoroughly
  2. Punch a 3 mm disc and place in a microcentrifuge tube
  3. Rinse punch in 500 μl water, pulse vortex 3 times for 5 seconds
  4. Remove water and centrifuge for 5 seconds; pipette off excess water
  5. Add 30 μl sterile water, heat at 95°C for 30 min; pulse vortex 60 times; centrifuge
  6. Use 5 to 10 μl eluted DNA in a PCR mixture

Use FTA Elute for a wide range of applications:

  • Multiplex PCR
  • Sequencing after PCR amplification
  • SNP analysis
  • STR analysis
  • Whole genome amplification
  • Quantitative PCR
  • Biobanking
  • Pharmacogenomics
  • Genotyping
  • Genetic identification
  • Transgenic detection

FTA Elute Micro Card 
Four sample application circles (11 mm diameter) per card, 40 µl sample per circle.

FTA Elute Sample Pack
Five FTA Elute Micro Cards, 3 mm Harris Uni-Core punch and instructions for use.

Indicating FTA Elute Micro Card
One sample application area (25 mm diameter) per card, 40 μl sample per circle.

Catalog Number     Description

WB120401     FTA Elute Micro Card with 4 sample areas per card, 25/pk
WB120410     FTA Elute Micro Card with 4 sample areas per card, 100/pk
WB120412     Indicating FTA Elute Micro Card with 1 sample area per card, 25/pk
WB120411     Indicating FTA Elute Micro Card with 1 sample area per card, 100/pk

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